Pioneering the next generation of diagnostic and therapeutic agents…

Students in the Chaput lab are working to develop the next generation of diagnostic and therapeutic agents based on novel synthetic genetic polymers that are capable of heredity and evolution. This is a highly interdisciplinary research environment that applies the principles of team science to combine such disparate fields organic chemistry, enzyme engineering, droplet microfluidics, and X-ray crystallography.

Current research interests include:

  • establishing new diagnostic platforms for disease detection & genotyping
  • discovering therapeutic aptamers that can compete with traditional antibodies
  • expanding the toolkit of enzymes available for synthetic biology

Chemical Synthesis

Chemistry SynthesisChemistry students in the lab are working to develop new types of XNA monomers and investigating chemical strategies for improving the functional properties of XNA aptamers and catalysts.

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Enzyme Engineering

Enzyme EngineeringEnzyme engineering students in the lab are using advanced microfluidics techniques to redesign the enzymes of life. We are primarily interested in establishing a broad toolkit of enzymes (e.g., polymerases, kinases, ligases, nucleases etc.) that the synthetic biology community can use to synthesize and modify synthetic genetic polymers with unique backbone structures (XNAs).

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Therapeutic & Diagnostic Agents

In Vitro SelectionMolecular biology students in the lab are evolving functional XNAs with specific target binding affinity (aptamers) and catalytic activity (XNAzymes). Functional XNAs isolated from these selections are basis for diagnostic and therapeutic applications.

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Structure Determination

Stucture AnalysisStructural biology students in the laboratory are working to solve the X-ray crystal structures enzymes developed by the protein engineering team. Examining how these enzymes function at a molecular level is necessary for understanding their mechanism of action, which in turn will help guide the design of new XNA enzyme variants that function with enhanced activity.

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